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PROGRAMMED CELL DEATH IN THE HONEY‐BEE ( APIS MELLIFERA L.) LARVAE MIDGUT
Author(s) -
GREGORC ALEŠ,
BOWEN IVOR D.
Publication year - 1997
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1997.0127
Subject(s) - midgut , acid phosphatase , endoplasmic reticulum , cytoplasm , microbiology and biotechnology , dna fragmentation , programmed cell death , lysis , biology , fragmentation (computing) , epithelium , alkaline phosphatase , nuclear membrane , biochemistry , apoptosis , botany , enzyme , larva , ecology , genetics
The histochemical and cytochemical localization of acid phosphatase has been used in an attempt to map the sites of cellular lysis and death. Reaction product was found both in the brush border of the midgut epithelium and in the basal membrane. Vacuolar acid phosphatase activity was found in the regenerative epithelial cells. Extra‐cisternal reaction product was associated with the endoplasmic reticulum which was dilated in lysed areas of the cytoplasm. Free acid and alkaline phosphatase activity was found in the basal area of the midgut epithelial cells and the former also occurred in the haemocoel. In the tracheoblastic cells only vacuolar acid phosphatase activity was seen. Chromatin aggregates were distributed throughout the nucleus and the nuclear envelope showed some infolding. Certain mature epithelial cells proved positive for anti‐histone associated DNA fragmentation indicative of programmed cell death.