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ALTERATIONS IN IMMUNOGLOBULIN SYNTHESIS DURING ADRIAMYCIN‐INDUCED APOPTOSIS IN MOUSE HYBRIDOMA CELLS
Author(s) -
MELIKSETIAN M. B.,
DAVTYAN T. K.,
CHIRKOWA I. V.,
ALEXANYAN YU.T.,
IGNATOVA T. N.
Publication year - 1997
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1996.0116
Subject(s) - apoptosis , antibody , chemistry , microbiology and biotechnology , cancer research , biology , immunology , biochemistry
Changes in the immunoglobulin synthesis in mouse hybridoma 1F 7 cell line following exposure to the anticancer drug adriamycin were studied. It was shown that adriamycin in concentrations up to 0.1μg/ml causes dose‐dependent reversible enhancement of immunoglobulin heavy γ 2b chain synthesis, and to a lesser extent, κ light chain synthesis. Enhancement of γ 2b heavy chain synthesis accompanies an increase in Ig γ 2b mRNA levels. Enhancement of DNA‐binding activity of another B‐cell specific protein, octamer transcription factor Oct‐2 was also observed. Cell cycle analysis revealed accumulation of the ADR‐treated cells in G 2 /M phase of cell cycle. These changes in hybridoma cells preceded the onset of internucleosomal DNA fragmentation and appearance of morphological characteristics of apoptosis. These findings suggest, that differentiation‐related processes occur during the latent phase of apoptosis induced by adria‐mycin in mouse hybridoma cells.