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SYNERGISTIC ANTITUMOUR ACTIVITY OF VITAMINS C AND K 3 AGAINST HUMAN PROSTATE CARCINOMA CELL LINES
Author(s) -
VENUGOPAL MEENAKSHI,
JAMISON JAMES M.,
GILLOTEAUX JACQUES,
KOCH JOHN A.,
SUMMERS MARCIA,
HOKE JASON,
SOWICK COLLEEN,
SUMMERS JACK L.
Publication year - 1996
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1996.0102
Subject(s) - catalase , oxidative stress , chemistry , cytotoxicity , dna synthesis , hydrogen peroxide , lipid peroxidation , thiol , vitamin c , cell culture , antioxidant , biochemistry , dna , microbiology and biotechnology , biology , in vitro , genetics
Vitamins C, K 3 (VC, VK 3 ) and a VC/VK 3 combination with a VC:VK 3 ratio of 100:1 were assayed for their antitumour activity against two human prostatic carcinoma cell lines. Co‐administration of the vitamins enhanced the antitumour activity 5‐ to 20‐fold even with a 1 h exposure time. While exogenous catalase destroyed the antitumour activity, hydrogen peroxide‐induced lipid peroxidation was negligible. Analysis of cellular ATP and thiol levels as well as DNA and protein synthesis revealed: a transient increase in ATP production, a decrease in DNA synthesis, an increase in protein synthesis and a decrease in thiol levels. These results suggested that the increased cytotoxicity of the vitamin combination was due to redox cycling and increased oxidative stress.