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ALBUMIN CAN REVERSE THE RELEASE OF POTASSIUM FROM HUMAN ERYTHROCYTES TREATED WITH THE NON‐IONIC DETERGENT, BRIJ 58
Author(s) -
BOGNER PETER,
WHEATLEY DENYS N.,
BORBÉLY CSABA,
MISETA ATTILA
Publication year - 1996
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1996.0096
Subject(s) - albumin , extracellular , chemistry , potassium , intracellular , incubation , metabolism , human albumin , biochemistry , sodium , human serum albumin , ionic bonding , bovine serum albumin , chromatography , biophysics , ion , biology , organic chemistry
Exchange of erythrocyte intracellular (i/c) K + for extracellular (e/c) Na + in human erythrocytes treated with sub‐CMC concentrations of the non‐ionic detergent Brij 58 can be stopped by reincubation in serum or albumin containing solutions. The progressive equilibration of the K + contents of detergent‐treated human erythrocytes with the incubation medium was reversed by an albumin‐mediated withdrawal of detergent molecules from the cell. Re‐establishment of near normal [K + ] in terms of K + /kg water proceeds in two ways: (i) a metabolism‐dependent net accumulation of K + ions; and (ii) a metabolism‐independent shrinkage of erythrocytes, this being the more significant factor.