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INDEPENDENT HIGH‐FREQUENCY OSCILLATIONS IN THE AMOUNTS OF INDIVIDUAL ISOZYMES OF LACTATE DEHYDROGENASE IN HL60 CELLS
Author(s) -
FERREIRA G. M. N.,
HAMMOND K. D.,
GILBERT D. A.
Publication year - 1996
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1996.0079
Subject(s) - isozyme , lactate dehydrogenase , biology , rhythm , period (music) , biochemistry , microbiology and biotechnology , enzyme , chemistry , medicine , physics , acoustics
Early studies, using kinetic methods, suggested that the isozyme pattern of lactate dehydrogenase in various cells oscillated with time. More recent electrophoretic studies on murine erythroleukaemic cells (which exhibit only one isozyme) indicated very high frequency variations (period 2min or less) in the amount of the lone active isozyme. We now show that in HL60 cells, the activity stain intensities of the two major isozyme bands both oscillate but the temporal variations are distinct. As with other cellular rhythms, each of the two periodicities seem to be modulated in cyclic fashion with respect to period, amplitude and mean levels, the periods of both the primary and modulating rhythms being of the order of 10–15min or probably much less.