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HIGH FREQUENCY OSCILLATIONS IN THE ACTIVITY OF PHOSPHOTYROSINE PHOSPHATASE IN MURINE ERYTHROLEUKAEMIC CELLS: ACTION OF INSULIN AND HEXAMETHYLENE BISACETAMIDE
Author(s) -
FERREIRA G. M. N.,
WÖLFLE H.,
HAMMOND K. D.,
GILBERT D. A.
Publication year - 1996
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1996.0078
Subject(s) - phosphatase , phosphorylation , inducer , mitosis , kinase , period (music) , chemistry , biology , microbiology and biotechnology , biochemistry , physics , acoustics , gene
We have previously reported oscillations in the activities of the phosphoamino acid phosphatases in murine erythroleukaemic cells. In keeping with our predictions we now show that the phosphotyrosine phosphatase activity rhythm has a much shorter period than originally seemed the case, being of the order of 10min and probably less. The periodic changes show evidence of rhythmic modulation of mean, period and amplitude as with all other cellular oscillations studied. Insulin decreases the frequency of the rhythm while the inducer of differentiation, hexamethylene bisacetamide (HMBA) decreases its amplitude. Current ideas on phosphorylation dynamics in relation to metabolism and mitosis may need to be revised in the light of the observations.