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CHARACTERIZATION OF FORCED SUSPENSION CULTURE OF SPONTANEOUSLY TRANSFORMED MOUSE FIBROBLASTS (B‐6)
Author(s) -
MATSUHISA TADAO,
TAKEMURA KOUICHI
Publication year - 1996
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1996.0056
Subject(s) - endoplasmic reticulum , golgi apparatus , suspension culture , intracellular , suspension (topology) , microbiology and biotechnology , mitochondrion , biology , chemistry , cell culture , genetics , mathematics , homotopy , pure mathematics
Mouse fibroblasts (B‐6) were cultured on agar‐coated dishes. After cells grew for 2–3 generations relatively rapidly in suspension, they began to grow very slowly (stationary phase). Electron microscopic studies showed that cells in a stationary phase developed intracellular organella: membranous structures (endoplasmic reticulum and Golgi apparatus) became manifest and the number of mitochondria increased. The specific activities of succinic‐cytochrome c reductase and 5′‐nucleotidase were three and five times higher, respectively, than those of cells on the dish.