SLOW INDUCTION OF GELATINASE B mRNA BY ACIDIC CULTURE CONDITIONS IN MOUSE METASTATIC MELANOMA CELLS | Zendy

KATO YASUMASA | Zendy; OZONO SATORU | Zendy; SHUIN TARO | Zendy; MIYAZAKI KAORU | Zendy

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SLOW INDUCTION OF GELATINASE B mRNA BY ACIDIC CULTURE CONDITIONS IN MOUSE METASTATIC MELANOMA CELLS

Author(s) -

KATO YASUMASA,

OZONO SATORU,

SHUIN TARO,

MIYAZAKI KAORU

Publication year - 1996

Publication title -

cell biology international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.932

H-Index - 77

eISSN - 1095-8355

pISSN - 1065-6995

DOI - 10.1006/cbir.1996.0044

Subject(s) - gelatinase , gelatinase a , cell culture , microbiology and biotechnology , melanoma , extracellular , reverse transcription polymerase chain reaction , extracellular matrix , matrix metalloproteinase , gene expression , biology , enzyme , messenger rna , chemistry , gene , cancer research , biochemistry , genetics

Gelatinase B has been thought to be a key enzyme for degradation of extracellular matrix in tumour invasion and metastasis. In this study, we examined the effect of acidic culture medium (pH5.9) on the expression of gelatinase B mRNA in mouse metastatic melanoma cell line (B16‐F10). Using reverse transcription‐polymerase chain reaction (RT‐PCR) analysis, we found that gelatinase B was induced by the acidic culture medium at 24h, and then gradually diminished to 72h. By gelatin zymographic analysis, gelatinase B was first detected at 24h, continued to increase and then reached a plateau at around 48h. These results suggest that the induction of gelatinase B secretion by acidic culture medium occurs as a result of the gene expression.

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