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SYNERGISTIC REGULATORY EFFECTS OF TNFα, IL‐1αAND IFNα ON THE GROWTH AND DIFFERENTIATION OF DAUDI LYMPHOMA CELLS
Author(s) -
SANTAVENERE E.,
PIETRO R.,
CENTURIONE M. A.,
DOMENICANTONIO L.,
TRUBIANI O.,
RANA R. A.
Publication year - 1996
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1996.0037
Subject(s) - tumor necrosis factor alpha , monoclonal antibody , cytokine , alpha (finance) , alpha interferon , lymphoma , receptor , cancer research , cell growth , microbiology and biotechnology , cell culture , interferon , cellular differentiation , chemistry , biology , immunology , antibody , medicine , biochemistry , gene , genetics , construct validity , nursing , patient satisfaction
The regulatory effects of the combined treatment of tumour necrosis factorα (TNFα), interleukin‐1α (IL‐1α) and interferonα(IFNα) on the growth and differentiation of Daudi lymphoma cells were investigated. By means of anti‐BrdU monoclonal antibodies and [ 3 H‐thymidine] incorporation a reduced proliferation rate was shown both through a combi‐nation of TNFα with either IL‐1α or IFNα and, above all, through simultaneous treatment with the three cytokines. In parallel, the degree of differentiation was evaluated via morphological criteria and detection of Fc receptors (FcR) and appeared higher after treatment with the three cytokines. Our results provide evidence of the increased sensitivity of this cell line to this combined treatment supporting the existence of a synergistic interaction in inducing the antiproliferative and differentiative effects.