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DISTRIBUTION OF ANNEXIN I DURING NON‐PATHOGEN OR PATHOGEN PHAGOCYTOSIS BY CONFOCAL IMAGING AND IMMUNOGOLD ELECTRON MICROSCOPY
Author(s) -
HARRICANE MARIECÉCILE,
CARON EMMANUELLE,
PORTE FRANÇOISE,
LIAUTARD JEANPIERRE
Publication year - 1996
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1996.0024
Subject(s) - immunogold labelling , pathogen , phagocytosis , confocal , electron microscope , confocal microscopy , microscopy , biology , microbiology and biotechnology , pathology , medicine , optics , physics
Abstract Annexin I is an abundant protein in U937 cells differentiated towards a macrophagic phenotype. These cells become able to kill Escherichia coli , however, the intracellular pathogen Brucella suis , known to interfere with phagosome maturation, multiply in these differentiated cells. We have analysed by confocal and electron microscopy the cellular localization of annexin I during phagocytosis of yeast, non‐pathogenic E. coli and the intracellular pathogen B. suis . Using immunocytochemical detections annexin I was found mainly as patches in the cytoplasm of uninfected cells. Upon phagocytosis of yeast or E. coli organisms, annexin I rapidly translocated and concentrated around phagosomes. On the other hand, annexin I was never detected around live B. suis ‐containing phagosomes. However, when dead brucellae were used, annexin I did translocate to the periphagosomal region. Our results suggest that annexin I could play a role in the molecular mechanism of phagosome maturation, which is impaired by some intracellular pathogens.