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COLLAGEN SYNTHESIS AND CELL GROWTH IN CHICK EMBRYO FIBROBLASTS: INFLUENCE OF COLCHICINE, CYTOCHALASIN B AND CONCANAVALIN A
Author(s) -
BODO M.,
CARINCI P.,
BARONI T.,
BECCHETTI E.,
BELLUCCI C.,
PEZZETTI F.,
GIAMMARIOLI M.,
STABELLINI G.,
ARE.
Publication year - 1996
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1996.0022
Subject(s) - colchicine , cytochalasin b , concanavalin a , cytochalasin , cytochalasin d , microbiology and biotechnology , embryo , receptor , biology , cytoskeleton , cell , chemistry , biochemistry , in vitro , genetics
Culturing of chick embryo fibroblasts in the presence of colchicine or cytochalasin B with and without concanavalin A (Con A) demonstrated that colchicine induces greater neosynthesis of endocellular type I collagen, whereas cytochalasin B boosts secretion. The effects are modified by the addition of Con A, which increases α 2 more than a1 chain production. 3 H‐thymidine incorporation is unaffected by cytochalasin B, but stimulated by colchicine. Con A neutralizes the stimulatory action of colchicine. It would therefore seem that Con A exerts transmembrane control of effects induced by colchicine and cytochalasin B by binding to cell surface receptors and so triggering rearrangement of the cytoskeleton.