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The subcellular localization of newly synthesized cytochrome b 5 .
Author(s) -
Carlsen Jens,
Christiansen Kirsten
Publication year - 1995
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1995.1127
Subject(s) - cytochrome , microsome , cytochrome b , compartment (ship) , mitochondrion , cytochrome c1 , heme , cytochrome c , biochemistry , chemistry , membrane , ribosome , hemeprotein , moiety , stereochemistry , coenzyme q – cytochrome c reductase , rna , enzyme , mitochondrial dna , oceanography , gene , geology
The fate of newly synthesized cytochrome b 5 was studied in rat hepatocytes. Using an antibody specific for microsomal cytochrome b 5 , we found newly synthesized microsomal cytochrome b 5 in both mitochondria and a mitochondria associated membrane fraction as well as in microsomes. Newly synthesized cytochrome b 5 was quickly removed from the site of synthesis on free ribosomes and inserted into membranes at random. No migration of newly synthesized cytochrome b 5 between cellular compartments was observed and therefore the assembly of the apoprotein with the heme moiety is apparently not taking place in any particular cellular compartment.