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Dye‐coupling between term pregnant human myometrial cells before labor: Carboxyfluorescein versus lucifer yellow
Author(s) -
Çiray H. Nadir,
Persson BoEric,
Roomans Godfried M.,
Ulmsten Ulf
Publication year - 1995
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1995.1108
Subject(s) - lucifer yellow , fluorescence , coupling (piping) , chemistry , gap junction , biophysics , anatomy , biology , microbiology and biotechnology , optics , materials science , intracellular , physics , metallurgy
Term pregnant human myometrial cells in whole mounts were microinjected by pressure with the fluorescent probes Lucifer Yellow and carboxyfluorescein. Tissues obtained from acute and elective sections displayed weak dye‐coupling when injected with Lucifer Yellow. Injection of carboxyfluorescein into cells from the elective sections resulted in a more extensive dye‐coupling than that observed with Lucifer Yellow. These results indicate that term pregnant human myometrial cells are metabolically coupled before labor and carboxyfluorescein is superior to Lucifer Yellow in detecting the coupling.