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Gene expression and protein distribution of inter‐α‐trypsin inhibitor in three human hepatoma cell lines.
Author(s) -
Héron Antoine,
Borghi Hélène,
Callé Aleth,
Bourguig Jeannette,
DiarraMehrpour Maryam,
Martin JeanPierre,
Sesboüé Richard
Publication year - 1995
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1995.1106
Subject(s) - trypsin , microbiology and biotechnology , cell culture , antiserum , chemistry , gene , trypsin inhibitor , hep g2 , gene expression , biology , biochemistry , antigen , in vitro , enzyme , genetics
In standard culture conditions, three human hepatoma cell lines, Hep3B, PLC/PRF/5 and HepG2, were characterised by a predominant transcription of only two (H2 and L) among the four genes involved in the synthesis of inter‐α‐trypsin inhibitor (ITI)‐related proteins. Pulse‐chase experiments followed by immunoprecipitation with specific anti‐L and anti‐H ITI antisera showed that the proteins synthesised displayed a restricted L and/or H2 antigenic reactivity. Furthermore, while Hep3B and PLC/PRF/5 lines only synthesised ITI precursors (mainly the L‐form), HepG2 cells were able to secrete an ITI‐like protein. Immunocytochemical analyses substantiated these results with uneven distribution of heavy and light‐chain polypeptide reactivity among the cells. The use of hepatoma cell models for the study of protein synthesis and assembly must therefore be considered cautiously.