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Early endocytotic steps in elicited macrophages: omega‐shaped plasma membrane vesicles at their cell surface.
Author(s) -
Kiss Anna L.,
Kittel Agnes
Publication year - 1995
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1995.1097
Subject(s) - endocytosis , macrophage , microbiology and biotechnology , population , cell , receptor mediated endocytosis , chemistry , vesicle , cell membrane , receptor , immune system , biophysics , biology , membrane , immunology , biochemistry , medicine , in vitro , environmental health
Abstract Fluid‐phase and receptor‐mediated endocytosis were studied in Freund's adjuvant elicited macrophages. These cells were found to bind and internalize significantly larger amounts of peroxidase‐antiperoxidase (PAP) immune complex than resident macrophages. Similarly the rate of the fluid‐phase uptake was higher in elicited cells. When studying the early steps of endocytotic processes, omega‐shaped plasma membrane pits (d∼90 nm) were found at the macrophage cell surface. Although occurring occasionally in resident cells, their number was highly increased after elicitation in 30% of the macrophage cell population. The different morphology of these cells coincided with a lower endocytotic activity and a very strong ecto Ca 2+ ‐ATPase reaction. The present findings indicate that the elicited macrophage population is heterogenous and consists of different subclasses.