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Uncoordinate expressions of type I and III collagens, collagenase and tissue inhibitor of matrix metalloproteinase 1 along in vitro proliferative life span of human skin fibroblasts
Author(s) -
BizotFoulon Valérie,
Bouchard Brigitte,
Hornebeck William,
Dubertret Louis,
Bertaux Brigitte
Publication year - 1995
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1995.1053
Subject(s) - collagenase , retinoic acid , messenger rna , in vitro , type i collagen , fibroblast , microbiology and biotechnology , life span , matrix metalloproteinase , matrix (chemical analysis) , human skin , biology , chemistry , cell culture , biochemistry , enzyme , endocrinology , gene , genetics , evolutionary biology , chromatography
In vitro human skin fibroblasts aging was characterized by a continuous increase of collagenase mRNA levels. On the contrary, TIMP‐1 mRNA level decreased only at late passages (> 65% of proliferative life span). Type I and III mRNA levels showed a high variability depending on cell strains studied. However, type I and III collagen expressions varied parallely. All‐trans retinoic acid (RA) decreased collagenase expression and stimulated TIMP‐1 expression. Under RA action, high variability in mRNAs levels encoding type I and III collagens was observed with HSF passages. However, RA tended to correct variations in collagens expressions observed along HSF life span.