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Proteins reacting with anti‐spectrin antibodies are present in Chlamydomonas Cells.
Author(s) -
Lorenz Michal,
Bisikirska Brygida,
HanusLorenz Beata,
Strzalka Kazimierz,
Sikorski Aleksander F.
Publication year - 1995
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1995.1048
Subject(s) - spectrin , chlamydomonas reinhardtii , immunoprecipitation , antibody , western blot , chlamydomonas , immunofluorescence , microbiology and biotechnology , chemistry , red blood cell , biology , biochemistry , cell , gene , cytoskeleton , immunology , mutant
It was found either in Western‐blot analysis or in indirect immunofluorescence microscopy that cells of the alga Chlamydomonas reinhardhi contain polypeptides cross‐reactng with antibodies directed against red blood cell spectrin. The protein could also be detected by immunoprecipitation with anti‐spectrin antibodies. C. reinhardtii cells contain distinct polypeptide chains reacting with antibodies directed against either α‐ or β‐ spectrin subunits. This protein was extracted from the cells with low ionic strength solution but was not with nonionic detergent.