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Microjet impingement followed by scanning electron microscopy as a qualitative technique to compare cellular adhesion to various biomaterials
Author(s) -
Richards R. Geoffrey,
Gwynn Ap Iolo,
Bundy Kirk J.,
Rahn Berton A.
Publication year - 1995
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1995.1044
Subject(s) - adhesion , cell adhesion , biocompatibility , biomaterial , scanning electron microscope , materials science , biomedical engineering , nanotechnology , microscopy , electron microscope , biophysics , chemistry , composite material , pathology , biology , medicine , optics , metallurgy , physics
Adhesion of cells to biomaterial surfaces is one of the major factors which mediates their biocompatibility. Quantitative or qualitative cell adhesion measurements would be useful for screening new implant materials. Microjet impingement has been evaluated by scanning electron microscopy, to determine to what extent it measures cell adhesion. The shear forces of the impingement, on the materials tested here, are seen to be greater than the cohesive strength of the cells in the impinged area, causing their rupture. The cell bodies are removed during impingement, leaving the sites of adhesion and other cellular material behind. Thus the method is shown not to provide quantification of cell adhesion forces for the metals and culture plastic tested. It is suggested that with highly adherent biomaterials, the distribution and patterns of these adhesion sites could be used for qualitative comparisons for screening of implant surfaces.