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No change in bone‐specific alkaline phosphatase activities in cultured rat osteoblastic cells under L‐ascorbate and β‐glycerophosphate‐induced mineralization
Author(s) -
Chak C. W.,
Lee K. M.,
Leung K. S.,
Fung K. P.
Publication year - 1995
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1995.1039
Subject(s) - mineralization (soil science) , alkaline phosphatase , chemistry , calcification , phosphate , biochemistry , von kossa stain , calcium , cell culture , intracellular , staining , endocrinology , medicine , biology , enzyme , organic chemistry , nitrogen , genetics
Mineralization occurred both in fetal rat calvarial cells and UMR 106 osteoblastic cells when they were cultured in medium containing L‐ascorbate and β‐glycerophosphate as evidenced by von Kóssa staining as well as deposition of calcium ions and inorganic phosphate in the cells. When compared with corresponding non‐mineralized cell cultures, both the mineralized cultures of calvarial cells and UMR 106 cells did not exhibit any change in intracellular bone‐specific alkaline phosphatase activities which were measured by wheatgerm lectin precipitation method. Our results support the hypothesis that mineralization may not exert any direct negative feedback on matrix protein synthesis in osteoblasts during bone formation.