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A confocal microscopic evaluation of the effects of insulin imprinting on the binding of Concanavalin A by Tetrahymena pyriformis .
Author(s) -
Kovcs Peter,
Sundermann Christine,
Estridge Barbara H.,
Csaba György
Publication year - 1995
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1995.1038
Subject(s) - tetrahymena pyriformis , concanavalin a , intracellular , tetrahymena , imprinting (psychology) , confocal microscopy , microbiology and biotechnology , biology , membrane , biophysics , cell , incubation , chemistry , biochemistry , in vitro , gene
With confocal microscopy it is possible to study the Concanavalin A (Con A) binding characteristics of the surface and interior of a single cell by viewing optical sections. It was observed in Tetrahymena pyriformis that Con A bound both to the plasma membrane and to intracellular structures. Incubation of cells with a competing sugar a‐methylmannopyranoside, decreased binding. Hormonal imprinting with insulin resulted in an increase in binding of Con A to the cell surface and a decrease in intracellular binding. It is possible that the intracellular binding sites may migrate to the plasma membrane.