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The DNA binding affinity of rat liver nucleoproteins to the regulatory elements of the haptoglobin and α 2 ‐macroglobulin genes.
Author(s) -
Petrović Miodrag,
Bogojević Desanka,
Grigorov Ilijana,
Ševaljević Ljiljana
Publication year - 1995
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1995.1037
Subject(s) - nucleoprotein , haptoglobin , gene , gene isoform , acute phase protein , macroglobulin , promoter , microbiology and biotechnology , biology , dna , transcription factor , regulatory sequence , chemistry , biochemistry , gene expression , endocrinology , immunology , inflammation
Transcriptional regulation and binding interactions between soluble nucleoproteins and the hormone response elements (REs) of the rat haptoglobin (Hp) and α 2 ‐macroglobulin (α 2 MG) genes was examined in the livers of rats during the acute‐phase reaction. Our results demonstrate that the elevation of the Hp and α 2 MG genes' transcription rates in acute‐phase liver relies essentially on an increase in the binding‐affinity of pre‐xisting soluble nucleoproteins, enhancing their capability to bind the examined cis‐regulatory elements. The 35kD nucleoprotein that displayed an acute‐phase inducible affinity to bind hormone REs of rat Hp and α 2 MG genes, was identified as a C/EBPβ isoform.