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Differences in the motility of Amoeba proteus isolated fragments are determined by F‐actin arrangement and cell nucleus presence
Author(s) -
Greȩbecka Lucyna,
Pomorski Pawel,
Łopatowska Anna
Publication year - 1995
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1995.1020
Subject(s) - nucleus , amoeba proteus , polarity (international relations) , actin , amoeba (genus) , biology , cell , biophysics , anatomy , microbiology and biotechnology , chemistry , biochemistry
Isolated fragments produced by bisection of Amoeba proteus differ by their position in the original cell and by the presence or absence of the cell nucleus. Immediately after the operation, both types of anterior fragments preserve the former motory polarity, and do not interrupt locomotion. In the same time, all posterior fragments stop, round up and fail to react stimuli. In the second phase of experiment, these anterior fragments, which had no nucleus ceased to move, whereas the nucleated posterior ones resumed locomotion. It was demonstrated, that the behaviour of a fragment is primarily determined by the peripheral F‐actin distribution, which is different depending on the origin of the fragment either from the anterior or from the posterior cell region. Later, the "inherited" F‐actin distribution may be stabilized or reorganized in the presence of the nucleus, or desorganized in its absence.