Premium
Secretion of TNF‐α from macrophages following induction with a lignin derivative.
Author(s) -
Sorimachi Kenji,
Akimoto Kazumi,
Tsuru Keiko,
Nagashima Shigeki,
Ieiri Tamio,
Niwa Akira
Publication year - 1995
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1995.1018
Subject(s) - tumor necrosis factor alpha , secretion , macrophage , blot , microbiology and biotechnology , messenger rna , giant cell , chemistry , alpha (finance) , biology , biochemistry , immunology , in vitro , medicine , gene , genetics , construct validity , nursing , patient satisfaction
Macrophages derived from rat bone marrow were treated with macrophage colony stimulating factor (M‐CSF) to obtain a sufficient number of cells for the tumor necrosis factor (TNF‐α) assay. The present study has been designed to investigate whether the production of TNF‐α, which induces multinucleated giant cell formation, is regulated by polyanions such as lignin derivatives. ELISA for TNF‐α showed that the polyanion induced TNF‐α production by macrophages. The secretion of TNF‐α from the cells reached a maximum at 3‐6 h, and then showed a slight decline. Northern blotting of TNF‐α mRNA showed that the amount of TNF‐α reached a maximum within 1 h of macrophage culture in the presence of a lignin derivative. On the other hand, TNF‐α mRNA was undetectable in the control cells. It was concluded that stimuli such as that provided by lignin derivatives increases the amount of TNF‐α mRNA, which is then followed by translation of TNF‐α.