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Polyamines and terminal deoxynucleotidyl transferase expression In KM 3 pre‐B cell line during phorbol ester induced differentiation.
Author(s) -
Stabellini Giordano,
Rapino Monica,
Primio Roberto,
Trubiani Oriana
Publication year - 1995
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1995.1016
Subject(s) - terminal deoxynucleotidyl transferase , putrescine , spermidine , microbiology and biotechnology , spermine , phorbol , intracellular , polyamine , biology , transferase , cell culture , biochemistry , dna synthesis , cellular differentiation , dna , tunel assay , enzyme , apoptosis , genetics , protein kinase c , gene
The aliphatic polyamines, putrescine, spermine and spermidine belong to a category of molecules implicated in DNA replication. Their synthesis is strongly activated during the G 1 period and they have been implicated in the regulation of cell proliferation and differentiation. Terminal transferase is a DNA polymerase present in pre‐T and pre‐B cells and its expression can be modulated by phorbol ester treatment. In this study we have monitored the relationship of intracellular polyamine levels with terminal deoxynucleotidyl transferase down‐regulation induced by 12‐0‐tetradecanoyl phorbol myristate 13‐acetate treatment in the human pre‐B KM‐3 cell line. Phorbol myristate acetate can cause an increase, at 4 and 8 hours of differentiation, of intracellular levels of putrescine as well as a decrease in terminal deoxynucleotidyl transferase synthesis showing the probable involvement that polyamines have in the differentiation process.