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Cycle delay in irradiated cytochalasin‐induced polykaryons. Does cytoskeleton status affect cell cycle checkpoints?
Author(s) -
Davies H. E.,
Court J. B.
Publication year - 1995
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1995.1003
Subject(s) - cytokinesis , microbiology and biotechnology , cytochalasin b , cytoskeleton , cell cycle , cytochalasin , biology , mitosis , cell , biophysics , cell division , genetics
Following exposure of CHO‐K1 cells to 137 Cs irradiation at doses up to 20Gy, a delay in G 2 was observed to occur in cells permitted to divide normally, while cells induced to become giants by means of cytochalasin B demonstrated a minimal delay in the transition 2C–8C suggesting that the inhibition of cytokinesis results in modification of one or more cell cycle checkpoints. We postulate that this may occur as a consequence of damage tolerance, or by a feedback loop resulting from the reorganisation of the cytoskeleton that precludes cytokinesis.