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Reversible macrophage differentiation induced in a new human myeloid cell line by gamma interferon.
Author(s) -
Pintér Claudia,
Magnani Ivana,
Paini Cinzia,
Clivio Alberto
Publication year - 1995
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1995.1002
Subject(s) - microbiology and biotechnology , biology , phenotype , signal transduction , myeloid , cell culture , interferon , macrophage , myeloid leukemia , cell , cell growth , cellular differentiation , interferon gamma , immunology , cytokine , in vitro , gene , genetics
A new cell line was established from the bone marrow of a patient with chronic myeloid leukemia. The cells were attributed an intermediate myeloid phenotype on the basis of their cytochemical features and membrane antigen expression. These cells respond to both chemical and physiological activators of the signal transduction pathways with growth arrest and phenotype changes. Macrophage maturation can be induced in a fraction of the cells by gamma‐interferon (γ‐IFN). Cells are however recruited again into the cell cycle by recultivation in γ‐IFN‐free medium: variants unresponsive to γ‐IFN, and others which show either reversible or irreversible differentiation were isolated from the original cell line by cloning and sib‐selection. These clones can be used to investigate the relationship between γ‐IFN response pathways and cell proliferation.