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Effect of extracellular K + on hatching and outgrowth of mouse blastocysts in vitro .
Author(s) -
Jin Zhengzhu,
Jin Meishan,
Roomans Godfried M.
Publication year - 1994
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1994.1127
Subject(s) - blastocyst , hatching , oviduct , in vitro , andrology , extracellular , embryo , biology , embryo culture , embryogenesis , chemistry , microbiology and biotechnology , biochemistry , medicine , zoology
The dependence of blastocyst development on the extracellular Na + /K + ratio was investigated in an in vitro system. Hatching and outgrowth of mouse blastocysts was enhanced at Na + /K + ratios between 3 and 10 compared to the ratio of about 25 typical for most culture media and serum. At a Na + /K + ratio of 2, blastocyst hatching and outgrowth were inhibited. The requirement of blastocyst development for relatively high extracellular K + concentrations agrees with the fact that K + concentrations in oviduct and uterine secretions are higher than in serum. The findings can also be relevant in optimizing in vitro culturing techniques for blastocysts.