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Immunohistochemical characterization of a stage‐specific antigen during oogenesis and spermatogenesis recognized with monoclonal antibody
Author(s) -
Itoh Masaaki,
Kimura Junpei,
Tsukise Azuma,
Okano Masaomi
Publication year - 1994
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1994.1115
Subject(s) - monoclonal antibody , immunohistochemistry , antigen , spermatogenesis , oogenesis , biology , antibody , stage (stratigraphy) , microbiology and biotechnology , pathology , immunology , medicine , oocyte , endocrinology , embryo , paleontology
In order to detect and characterize novel molecules which function in oogenesis, immunohistochemical study using a monoclonal antibody (MAb) raised against oocytes of Xenopus laevis was carded out. The distribution of the detectable molecule with MAb X‐80, which specifically reacts with Xenopus previtellogenic oocytes; stages I and II (Dumont, 1972), in particular was analyzed. The hamster, mouse, chick, quail, Caenorhabditis elegans and Lilium longiflorum were also examined. Interestingly MAb X‐80 bound not only in Xenopus oocytes but in its secondary spermatocytes and spermatids. Furthermore, MAb X‐80 gave a similar staining pattern in the Lilium and other, although the stage when the positive reaction is detectable is different in the male and female germ cells.