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Benzamide‐induced changes in the actin cytoskeleton in human skin fibroblasts.
Author(s) -
Korohoda W.,
Michalik M.,
Pierzchalska M.
Publication year - 1994
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1994.1111
Subject(s) - benzamide , microtubule , actin , chemistry , cytoskeleton , incubation , microbiology and biotechnology , biophysics , human skin , cell , biochemistry , biology , stereochemistry , genetics
The incubation of human skin fibroblasts in the presence of 10 mM benzamide in Joklik's modification of Eagle's Minimal Essential Medium caused an extensive reorganization of actin filaments. The disappearance of stress fibers and changes in cell morphology were observed, whereas no changes in the microtubule architecture were noticed. The observed effects appeared fully reversible within 3 hours after the removal of benzamide. The results are discussed in relation to the two known activities of benzamide as an anaesthetic and an inhibitor of ADP‐ribosylation.