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Neutrophil polarisation in plasma differs to that induced by endogenous chemoattractants with regard to frequency of uropod formation and requirement for divalent cations.
Author(s) -
Harkin Damien G.,
Bignold Leon P.
Publication year - 1994
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1994.1059
Subject(s) - divalent , extracellular , chemotaxis , biophysics , endogeny , salt (chemistry) , chemistry , leukotriene b4 , mediator , microbiology and biotechnology , biochemistry , biology , receptor , inflammation , immunology , organic chemistry
Human neutrophils suspended in Hanks' balanced salt solution (37° C, 20 mM Hepes, pH 7.2) produced extensions, elongated and developed a polarised morphology with both a pseudopod and uropod when exposed to C5a (10 nM), leukotriene B4 (10 nM), platelet activating factor (40 nM) or interleukin‐8 (12.5 nM). Responses to each mediator were generally enhanced or unaffected by chelators of extracellular Ca 2+ and Mg 2+ . Neutrophils suspended in heparinised plasma (90‐10% v/v in Hanks' balanced salt solution) produced extensions, elongated and developed a pseudopod, but rarely developed a uropod unless additional Mg 2+ ions (0.5‐5 mM) were added. These findings demonstrate that the polarisation of neutrophils in plasma is significantly different to that induced by endogenous chemoattractants with regard to the frequency of uropod formation and requirement for extracellular divalent cations.