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Tumor necrosis factor alpha, a cytokine coregulating sugar uptake by cultured human synovial cells.
Author(s) -
Hernvann Alain,
SouliacMarc Catherine,
Aussel Christian,
Ekindjian Ohvanesse
Publication year - 1994
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1994.1056
Subject(s) - cytokine , tumor necrosis factor alpha , stimulation , endocrinology , prostaglandin e2 , synovial membrane , cell culture , medicine , rheumatoid arthritis , chemistry , immunology , biology , genetics
Confluent cultures of osteoarthritic and rheumatoid human synovial cells were treated with human recombinant tumor necrosis factor alpha (TNF‐α) The cytokine increased uptake of 2‐deoxy‐D‐[1‐ 3 H]glucose (2‐DOG) in a time‐ and concentrations‐dependent manner. In synovial cells obtained from osteoarthritic patients (OA cells), the stimulation of 2‐DOG uptake occurred 3 hours following addition of TNF‐α (1 ng/ml) and was maximal by 24 hours Rheumatoid synovial cells (RA cells) appeared less sensitive to the cytokine: 2‐DOG uptake stimulation was only significant after 6 hours of incubation. In both OA and RA cells, the effect was protein synthesis‐dependent, and was not secondary to prostaglandin E2 synthesis or cell growth. Interteukin‐1β was more efficient than TNF‐α for 2‐DOG uptake stimulation The two cytokines seemed to act in an additive manner.