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Studies on bcl‐2 protein in cultured lymphoid cells.
Author(s) -
Filip Agata,
Rożynkowa Danuta
Publication year - 1994
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1994.1048
Subject(s) - biology , staining , microbiology and biotechnology , progenitor cell , oncogene , lymphatic system , pathology , cell , chemistry , immunology , stem cell , cell cycle , biochemistry , medicine , genetics
Bc1‐2 protein was analysed in cell cultures derived from non‐Hodgkin lymphomas and chronic lymphocytic leukaemia patients. The presence of bc1‐2 proto‐oncogene protein product has been demonstrated on cytospin preparations utilising immunochemical methods. The correlation between the expression of the bc1‐2 protein and the appearance of proliferation‐ or differentiation related figures was studied. The reported bc1‐2 up regulation in malignant lymphoid cells was confirmed in haemo‐ and lymphopoietic progenitor cells as well as in TPA/PHA activated peripheral blood lymphocytes. In comparison to neoplastic cells lower intensity of bc1‐2 positive staining in freshly isolated cells from non‐leukaemic tissue was observed.