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Effects of hypothermia on the survival and cryopreservation of minipig ileal cells and Chinese hamster ovary cells.
Author(s) -
Kaeffer B.,
Uriel I. Garcia,
Bottreau E.
Publication year - 1994
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1994.1029
Subject(s) - hypothermia , cryopreservation , biology , chinese hamster ovary cell , andrology , cell culture , cytokeratin , ovary , hamster , cell , immunology , microbiology and biotechnology , embryo , endocrinology , biochemistry , medicine , immunohistochemistry , physiology , genetics
Temperature of culture can be used to modulate cellular metabolism for improving small intestinal cell culture and cryopreservation. An hypothermia pretreatment (2 days at 25°C and 3 hours recovery at 37°C) improved hamster cell survival to freeze‐thaw damage (p < 0.01) but decreased the survival of 2 immortal pig ileal cell lines even though epithelioid IPI‐2I cells were more tolerant to hypothermia than IPI‐I fibroblasts. Epithelioid cells survived 3 days at 25°C with unaltered expression of cytokeratin‐18 whereas colonies of fibroblasts did not survive more than a day at 25°C (p < 0.001). These results suggest that hypothermia‐tolerance of pig ileal cell lines might differ according to cell lineage calling for further experiments on small intestinal primary cell culture.