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Proliferation of human leukemic pre‐B cells induced by human bone marrow stromal cells and murine fibroblasts.
Author(s) -
Buske Christian,
Hiddemann Wolfgang,
Wörmann Bernhard
Publication year - 1994
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1994.1028
Subject(s) - stromal cell , bone marrow , cell culture , in vitro , fibroblast , cytokine , microbiology and biotechnology , biology , cancer research , human bone , cell growth , immunology , genetics
Aim of the study was the establishment of a standardized in vitro culture system for studies on proliferation and differentiation of human leukemic pre ‐ B cells. Coincubation with human stromal cells led to a significantly higher proliferation of the cytokine ‐ sensitive leukemic pre ‐ B cell line BLIN‐1. Clones from the murine fibroblast cell line L929 provided identical results. Coincubation with the murine cells also resulted in a significantly higher numbers of viable cells in 5 of 8 patient samples with newly diagnosed B lineage ALL. The results show that in vitro bone marrow stromal cells can be substituted by murine fibroblasts and form the basis for a simpler and more reproducible assay system.