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4‐Hydroxynonenal induces membrane perturbations and inhibition of basal prostacyclin production in endothelial cells, and migration of monocytes.
Author(s) -
Moldovan Nicanor I.,
Lupu Florea,
Moldovan Leni,
Simionescu Nicolae
Publication year - 1994
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1994.1020
Subject(s) - prostacyclin , chemotaxis , phospholipid , chemistry , peripheral blood mononuclear cell , medicine , microbiology and biotechnology , biology , endocrinology , biochemistry , in vitro , membrane , receptor
Cultured bovine aortic endothelial cells (BAEC) were incubated for 5 days with 10 −5 4‐hydroxynonenal (HN). HN treated BAEC and controls were either (i) further incubated with 125 I‐polymyxin B (IPxB) or with radioiodinated, inactivated coagulation factor Xa (IFXai) as markers of membrane phospholipid perturbation, or (ii) assayed for the synthesis of prostacyclin (PGI 2 ) and thromboxane A 2 (TXA 2 ). Rabbit blood mononuclear cells enriched in monocytes (MC) were isolated and assayed for chemotactic response to HN. The results showed six ‐ fold increases of IPxB and IFXai binding to BAEC treated with HN, as compared to untreated controls. We also found in HN treated cells a marked inhibition of PGI 2 synthesis, but an unmodified TXA 2 production. In addition, HN in the 10‐5‐10‐10 M range induced oriented migration of MC.