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Gene regulation in intertypic heterokaryons of Solanum tuberosum and Nicotiana tabacum tissue protoplasts
Author(s) -
Kesteren W. J. P.,
Bijmolt E. W.,
Tempelaar M. J.
Publication year - 1994
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1994.1014
Subject(s) - protoplast , nicotiana tabacum , heterokaryon , biology , solanum tuberosum , cauliflower mosaic virus , solanaceae , chimeric gene , reporter gene , transgene , gene , microbiology and biotechnology , gene expression , genetically modified crops , botany , biochemistry , mutant
Activities of the ß‐glucuronidase (GUS) reporter enzyme were evaluated in transgenic plants, protoplasts, and intertypic heterokaryons of Solanum tuberosum and Nicotiana tabacum . With GUS under control of the promoter of the cauliflower‐mosaicvirus 35S RNA gene (CaMV), activities of the enzyme were nearly evenly distributed over the tissues of plants grown in vitro . Activities in microtubers of potato plants with the reporterenzyme under control of the promoter of granule‐bound starch synthase (GBSS) from S. tuberosum were higher than in leaves. The CaMV‐GUS construct present in leaf protoplasts showed an increased expression in biochemical and cytochemical assays after fusion with wildtype‐tuber protoplasts. Such an increase was not observed in the case of the GBSS‐GUS constructs. It was concluded that nuclei in plant heterokaryons are being influenced by the fusion partner, but that repressive, trans ‐acting regulation factors in leaf protoplasts possibly prevent an increase of the expression of the chimeric GBSS‐gene in heterokaryons.