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Transfected Chinese hamster ovary cells as a model system for cytokine immunocytochemistry and in situ hybridisation.
Author(s) -
Mueller Regula,
Junker Udo,
Wagner Kathrin,
Heusser Christoph,
Bullock Gillian
Publication year - 1994
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1994.1007
Subject(s) - chinese hamster ovary cell , immunocytochemistry , cytokine , monoclonal antibody , biology , hamster , population , transfection , antibody , in situ hybridization , in situ , immunology , cell culture , microbiology and biotechnology , chemistry , medicine , biochemistry , endocrinology , gene expression , gene , genetics , environmental health , organic chemistry
The presence of cytokine producing cells is most easily revealed by techniques measuring the secreted cytokines in culture supernatants or body fluids. However, these techniques only measure the bulk cytokine release by a given, often mixed cell population. To demonstrate cytokine production at the single cell level, immunocytochemistry (ICC) and in situ hybridisation (ISH) are now widely used techniques. To establish these techniques, an easily accessible model system is needed which permits the evaluation of different ICC and ISH protocols. It can be used to demonstrate the specificity of the antibodies and may serve as a positive control for samples of unknown cytokine content. Here we propose the use of Chinese hamster ovary (CHO) cells transfected to express one specific cytokine as such a model system. Its usefulness is demonstrated by the characterisation of six monoclonal antibodies to human interleukin‐4 and the establishment of two in situ hybridisation protocols.