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Production of the metastatic phenotype by DNA transfection in a rat mammary model.
Author(s) -
Davies Barry R.,
Barraclough Roger,
Davies Michael P. A.,
Rudland Philip S.
Publication year - 1993
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1993.1150
Subject(s) - transfection , biology , cell culture , cancer research , oncogene , microbiology and biotechnology , mammary tumor , phenotype , complementary dna , mammary gland , metastatic breast cancer , gene , cancer , cell cycle , breast cancer , genetics
Abstract A syngeneic, immunocompetent rat mammary model has been employed to investigate the molecular basis of the metastatic phenotype. Transfection of the benign rat mammary epithelial cell line Rama 37 with the gene for p9Ka; a small, rat, calcium‐binding protein or DNA from metastatic human cell lines derived from a primary breast carcinoma or a pleural effusion yields transfectants with metastatic capabilities. Transfection of DNA from a benign human mammary cell line, a plasmid containing a cDNA for the human basic fibroblast growth factor (bFGF) gene, or the oncogene EJ‐ras‐1, fails to yield any transfectants expressing the metastatic phenotype.