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A comparison of bovine serum albumin and chicken ovalbumin as supplements for the serum‐free growth of chinook salmon embryo cells, CHSE‐214.
Author(s) -
Barlian A.,
Ganassin R. C.,
Tom D.,
Bols N. C.
Publication year - 1993
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1993.1118
Subject(s) - bovine serum albumin , trypsin , ovalbumin , egg white , embryo , biology , albumin , cryopreservation , biochemistry , chemistry , andrology , enzyme , immunology , microbiology and biotechnology , antigen , medicine
Chicken ovalbumin and bovine serum albumin (BSA) were compared as supplements to the basal medium, L‐15, for the serum‐free cultivation of the Chinook Salmon Embryo cell line, CHSE‐214. Unlike L‐15 alone, ovalbumin and some commercial BSA preparations allowed cell proliferation and development of confluent monolayer cultures. However, only a fatty acid‐free BSA (2%) supported continuous proliferation for two years through approximately 15 subcultivations. For this, subcultivation was achieved with non enzymatic cell dissociation solutions. Also, new serum‐free subcultivation techniques were developed that utilized avian egg white trypsin inhibitors to terminate the action of either bovine or cod trypsin. Finally, CHSE‐214 were successfully cryopreserved in 2% BSA, allowing all cell cultivation steps to be performed in the absence of FBS.