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Absence of high levels of DNA polymerase α activity in the nuclear matrix prepared from mouse erythroleukemia cells
Author(s) -
Martelli Alberto M.,
Neri Luca M.,
Billi Anna Maria,
Bareggi Renato,
Cocco Lucio
Publication year - 1993
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1993.1113
Subject(s) - nuclear matrix , dna polymerase , polymerase , dna , microbiology and biotechnology , matrix (chemical analysis) , chemistry , dna clamp , nuclear dna , extraction (chemistry) , incubation , dna replication , dna synthesis , biology , polymerase chain reaction , biochemistry , chromatography , reverse transcriptase , gene , chromatin , mitochondrial dna
Abstract We have examined the association of DNA polymerase α activity with the nuclear matrix prepared by different techniques from mouse erythroleukemia cells. At variance with the data obtained using other cell types we have found that only a small amount (less than 2%) of nuclear DNA polymerase α activity resisted extraction with high‐ionic strength buffers, even if nuclei were heat‐stabilized by incubation at 37°C for 45 min prior to subfractionation. The recovery of DNA polymerase α activity bound to the matrix was unaffected by the type of extracting agent used (NaCl or (NH 4 ) 2 SO 4 ), by the extraction sequence or by the method employed for obtaining nuclei. These results could indicate that in some types of cells the nuclear matrix is not involved in DNA replication.