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Combined biochemical and immunocytochemical analyses of postmortem protein deimination in the rat spinal cord
Author(s) -
Asaga Hiroaki,
Senshu Tatsuo
Publication year - 1993
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1993.1094
Subject(s) - citrullination , citrulline , spinal cord , immunocytochemistry , cytoplasm , chemistry , staining , in vitro , nervous tissue , endogeny , biochemistry , enzyme , microbiology and biotechnology , arginine , pathology , biology , amino acid , medicine , neuroscience
Immunocytochemical staining of citrulline‐containing proteins, i.e. products of endogenous peptidylarginine deiminase (EC 3.5.3.15) reaction, has been performed by chemical modification of citrulline residues in situ , followed by probing with IgG specific to the modified residues. Rat spinal cords that had been preincubated in vitro to accelerate the enzyme reaction were used as samples. Not only astrocytes but also the cytoplasm of some large neurons were stained. Usefulness of the method for studying nervous tissue damage was suggested.