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Relationship between cell adherence and proteoglycan synthesis in cultures of human peripheral blood mononuclear cells: effects of concanavalin A.
Author(s) -
Anastassiades T. P.,
Chopra R. K.,
Ford P. M.,
Wood A.
Publication year - 1993
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1993.1091
Subject(s) - concanavalin a , peripheral blood mononuclear cell , chondroitin sulfate , incubation , population , proteoglycan , microbiology and biotechnology , stimulation , cell culture , monoclonal antibody , biology , cell , chondroitin sulfate proteoglycan , antibody , biochemistry , chemistry , immunology , endocrinology , in vitro , medicine , extracellular matrix , glycosaminoglycan , genetics , environmental health
Human, blood‐derived mononuclear cells (MC), stimulated with Concanavalin A (Con A), synthesized a chondroitin sulfate (CS) proteoglycan (PG), which was elaborated largely by T‐cells. Following Con A stimulation, the majority of MC adhered to the culture dish by day 2; but as incubation progressed to day 10 the proportion of non‐adherent (NAd) MC increased in a fashion which approximately paralleled the accumulation of PG in the medium. Cell kinetic studies suggest that, following Con A stimulation, there was an inverse relationship between the amount of newly synthesized cellular PG and adherence, which appears to be related to a reciprocal effect on PG synthesis of the declining adherent (Ad) cell density with time of culture. In the stimulated cultures, NAd cells contained much more newly synthesized CS/cell than Ad cells up to day 6 of incubation. Cell type analysis, using monoclonal antibodies against specific cell surface markers, suggested that the higher PG synthesis in the NAd population may, at least in part, be due to a greater proportion of T‐helper cells.