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The increase in cytosolic levels of protein kinase C precedes the growth factor requirements during the transition stem cells to differentiated progeny in the MCF‐7 breast tumor cells.
Author(s) -
Gomez Maria L.,
Resnicoff Mariana,
Medrano Estela E.,
TellezI Maria T.
Publication year - 1993
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1993.1090
Subject(s) - biology , stem cell , mcf 7 , microbiology and biotechnology , cytosol , protein kinase c , context (archaeology) , cellular differentiation , mitosis , kinase , gene , enzyme , genetics , biochemistry , human breast , cancer cell , paleontology , cancer
The expression of regulatory genes within the context of a differentiation program can have profound long‐term consequences in tissues with permanent renewing populations. The breast tumor cell line MCF‐7 retains in culture some of the characteristics of a unidirectional differentiation pathway. We show that the cytosolic activity of the regulatory enzyme protein kinase C (PKC) precedes and continues the sequence of maturation in pre‐differentiated subpopulations derived from a stem cell fraction. However, the activity declines in the most differentiated, post‐mitotic fraction. These results indicate that PKC may be considered among the regulatory genes in MCF‐7 cells that specify maturation of the stem cell progeny.