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Transforming growth factor(TGF)‐β1 induces leukemic cell‐growth‐promoting activity in fibroblast cells.
Author(s) -
Komatsu K.,
Nakamura H.,
Akedo H.
Publication year - 1993
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1993.1082
Subject(s) - fibroblast , transforming growth factor , secretion , cell culture , microbiology and biotechnology , cell growth , biology , glycoprotein , transforming growth factor beta , chemistry , biochemistry , genetics
The production of the leukemic cell‐growth‐promoting factor (LGF) in TGF‐β1‐treated fibroblast cells was studied. BALB/c3T3 mouse fibroblast(3T3) cells cultured in Eagle's medium containing a low concentration of TGF‐β1 (0.04‐1 ng/ml) secreted 3‐5 times more LGF than the cells cultured in the absence of TGF‐β1. The amount of LGF secretion was dose‐dependent on the concentration of post‐cultured medium and time‐dependent after the addition of TGF‐β1. Similar findings were obtained in human diploid fibroblasts, WI‐38 cells. LGF is a 18KD glycoprotein that is acid‐stable but heat‐unstable.