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Study of localization of the protein‐synthesizing machinery along actin filament bundles.
Author(s) -
Shestakova Elena A.,
Motuz Ludmila P.,
Minin Alexander A.,
Gavrilova Lydia P.
Publication year - 1993
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1993.1079
Subject(s) - microfilament , microbiology and biotechnology , actin , cytoskeleton , biology , cytochalasin b , protein filament , ribosome , biophysics , rna , biochemistry , in vitro , cell , gene
Indirect immunofluorescent microscopy was used to study the distribution of elongation factor 2 (eEF‐2) in fixed human skin diploid and mouse embryo fibroblasts. It was found earlier that some of the eEF‐2 ribosomes and initiation factor 2 (eIF‐2) are co‐localized with a part of the actin microfilament bundles in these cells (Gavrilova et al., 1987; Shestakova et al., 1991). Here it has been shown that inhibition of protein synthesis either by inactivation of eEF‐2 itself with diphtheria toxin or by inactivation of ribosomes with ricin does not abolish the distribution of eEF‐2 along the actin microfilament bundles. At the same time, the disassembly of actin microfilaments by cytochalasin D results also in the disappearance of eEF‐2‐carrying threads. This means that the eEF‐2‐carrying threads do not exist per se, and that the organization of eEF‐2 in visible "filaments" depends upon the integrity of the actin cytoskeleton.