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Effect of TGF‐Alpha on growth of normal human breast epithelial cells in serum‐free primary culture using 3‐dimensional collagen gels.
Author(s) -
Sakthivel Ramasamy,
Hamdan Maha,
Yang Jason,
Guzman Raphael C.,
Nandi Satyabrata
Publication year - 1993
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1993.1077
Subject(s) - endocrinology , transforming growth factor , medicine , stimulation , fibroblast growth factor , cholera toxin , tgf alpha , alpha (finance) , chemistry , cell culture , growth factor , biology , receptor , construct validity , genetics , nursing , patient satisfaction
The mitogenic effect of TGF‐alpha, acidic‐FGF, basic‐FGF and lithium on normal human breast epithelial cells was studied in a collagen gel culture system using a serum‐free 1:1 mixture of Ham's F12 and DME medium containing insulin, cholera toxin and bovine serum albumin. TGF‐alpha elicited a strong mitogenic response in a dose dependent manner. Addition of cortisol to TGF‐alpha stimulated growth over and above that achieved with TGF‐alpha alone. A consistent observation has been the effect of a combination of TGF‐alpha and cortisol on growth stimulation of normal human breast epithelial cells resulting in 3‐12 fold growth after 11‐13 days in culture. Acidic‐FGF, basic‐FGF and lithium were not growth promoting.