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Effect of enthidium on the morphology, antiviral activity and subcellular distribution of Poly r(A‐U)
Author(s) -
Jamison James M.,
Gilloteaux Jacques,
Adrian Marc,
Summers Jack L.
Publication year - 1993
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1993.1042
Subject(s) - ethidium bromide , chemistry , microbiology and biotechnology , confocal microscopy , confocal , biophysics , ultrastructure , fluorescence , interferon , cytotoxicity , biology , biochemistry , virology , dna , in vitro , anatomy , physics , geometry , mathematics , quantum mechanics
When ethidium bromide (EB) is combined with poly r(A‐U) at an EB/ribonucleotide ratio of 1/4, the antiviral activity of the EB increases 22‐fold. The increased antiviral activity is not due to increased interferon induction, direct viral inactivation or host cell cytotoxicity. Phase contrast, confocal and fluorescence microscopic observations reveal an increase in the nucleolar accumulation of the EB and/or the poly r(A‐U) in the EB/poly r(A‐U)‐treated fibroblasts. Ultrastructure of negatively stained and replica preparations demonstrated that EB‐induced condensation of poly r(A‐U). These results suggest the elevated antiviral activity may be related to the altered uptake and subcellular distribution of the EB/poly r(A‐U) complex.