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The effect of insulin on proteins associated with free, cytoskeletal‐bound and membrane‐bound polysome populations.
Author(s) -
Moss Rigmor,
Pryme Ian F.,
Vedeler Anni
Publication year - 1993
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1993.1039
Subject(s) - polysome , cytoskeleton , stimulation , insulin , ribosome , biology , protein biosynthesis , cytosol , membrane protein , biochemistry , microbiology and biotechnology , membrane , chemistry , endocrinology , rna , cell , enzyme , gene
It is known that insulin treatment increases the rate of protein synthesis in many cells and tissues and that it causes changes in the distribution of ribosomes between free (FP), cytoskeletal‐bound (CBP) and membrane‐bound polysome (MBP) populations. This paper concerns an analysis of the pattern of proteins in high‐salt extracts of FP, CBP and MBP isolated from Krebs II ascites and MPC‐11 cells. A combined detergent/salt extraction procedure was used to isolate the three fractions of polysomes from control cells and from cells following short‐term stimulation with insulin. There were differences in the protein patterns in the individual fractions and changes occurred after insulin stimulation.