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Retraction of cultured endothelial cell monolayer by human breast cancer cells, MCF‐7.
Author(s) -
Yoshioka Kiyoko,
Mukai Mutsuko,
Shinkai Kiyoko,
Akedo Hitoshi
Publication year - 1993
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1993.1038
Subject(s) - mcf 7 , human breast , cancer research , chemistry , monolayer , cancer cell , endothelial stem cell , cell , cancer , microbiology and biotechnology , biology , medicine , biochemistry , in vitro
A human breast cancer cell line (MCF‐7), when sealed on confluent bovine pulmonary aortic endothelial cell (CPAE) monolayers, induced morphological changes (retraction) in CPAE cells. The area of retraction depended on the incubation time and the number of MCF‐7 cells, suggesting that MCF‐7 cells had the capacity to retract CPAE cells. This capacity was reduced by 60% by pretreatment of MCF‐7 cells with 17β‐estradiol (E) and progesterone (Pg). The extent of retraction was not affected by the addition of various protease inhibitors. CPAE retraction was induced also by adding conditioned medium (CM) from the culture of MCF‐7 cells. Considerably less activity was detected in the CM obtained from MCF‐7 cells cultured in the presence of E and Pg. The retraction was reversed in 24 h by culturing the monolayer in fresh medium without CM and was not induced by trypsin treatment of the CM.