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PCNA independence of Ki‐67 expression in HPV infection.
Author(s) -
Boon M. E.,
Howard C. V.,
Velzen D.
Publication year - 1993
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1993.1029
Subject(s) - proliferating cell nuclear antigen , antigen , immunoperoxidase , virology , biology , staining , microbiology and biotechnology , immunohistochemistry , immunology , antibody , monoclonal antibody , genetics
12 Infectious Wart lesions were stained using the streptavidin‐biotin immunoperoxidase method for PCNA10, MIB‐1 (Ki‐67 equivalent antigen) and Human Papilloma Virus antigen to study the effect of HPV presence on epidermal proliferation. Using strict methods to avoid observer bias, Ki‐67 antigen was found in a high proportion of nuclei in the suprabasal layers together with HPV antigens, in the absence of PCNA staining. This finding indicates that DNA synthesis related, Ki‐67 antigen bearing structures can be raised in the human nucleus in the absence of induction of PCNA bearing structures, suggesting also structural independence between the antigen bearing molecules.